Fig. 6

Inhibition of Rac1 impaired the effect of MSC-CM on Na⁺ transport. FDLE cells were subjected to MSC-CM or control medium for 24 h with or without the Rac1 inhibitor. Data are displayed as boxes and whiskers with the 10–90 percentiles, mean (+) and median (horizontal line). Statistical differences among groups were analyzed with an ANOVA and Tukey’s post hoc test. a ∆I_amil was significantly reduced by Ehop-016 in FDLE cells cultured in MSC-CM. Controls were not affected by Rac1 inhibition (n = 38–44, 5 IE; ***p < 0.001). b R_te of FDLE cells in MSC-CM was decreased by Ehop-016 (*p < 0.05). c ∆V_amil was decreased by Ehop-016 in MSC-CM-treated cells, which prevented the MSC-CM-induced increase of ∆V_amil (***p < 0.001). □ control, ■ MSC-CM