Fig. 7

PD-MSC^PRL-1 coculture activates mitochondrial dynamics of primary hepatocytes. Protein expression of a albumin and PRL-1 and b mitochondrial metabolism (PDH and ATP5B) by naïve or PD-MSC^PRL-1 coculture with or without siRNA-PRL-1 (siPRL-1; 50 nM) for 24 h in primary hepatocytes treated with lithocholic acid (LCA; 100 μM) for 12 h by western blotting. c mRNA expression of mitochondrial dynamics (PGC-1α, NRF1, and mtTFA) by qRT-PCR analysis. d mtDNA copy number in primary hepatocytes by the TaqMan assay. e ATP production analysis in primary hepatocyte lysate (10 μg/μl). Data from each group are presented as the mean ± SD. ^#p < 0.05 versus control (−); ^*p < 0.05 versus coculture groups; ^**p < 0.05 versus naïve; ^$p < 0.05 versus NC. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; mtTFA, mitochondrial transcription factor A; NC, negative control; NRF1, nuclear respiratory factor 1; PDH, pyruvate dehydrogenase; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator-1 alpha; PRL-1, phosphatase of regenerating liver-1