Table 1 Primer sequences using reverse transcription polymerase chain reaction
Genes | Â | Primer sequences | Tm |
|---|---|---|---|
Oct4 | Forward | 5′-AGTGAGAGGCAACCTGGAGA-3′ | 52 |
Reverse | 5′-GTGAAGTGAGGGCTCCCATA-3′ | ||
Nanog | Forward | 5′-TTCTTGACTGGGACCTTGTC-3′ | 52 |
Reverse | 5′-GCTTGCCTTGCTTTGAAGCA-3′ | ||
Sox2 | Forward | 5′-GGGCAGCGTGTACTTATCCT-3′ | 52 |
Reverse | 5′-AGAACCCCAAGATGCACAAC-3′ | ||
HLA-G | Forward | 5′-GCGGCTACTACAACCAGAGC-3′ | 58 |
Reverse | 5′-GCACATGGCACGTGTATCTC-3′ | ||
TERT | Forward | 5′-GAGCTGACGTGGAAGATGAG-3′ | 55 |
Reverse | 5′-CTTCAAGTGCTGTCTGATTCCAATG-3′ | ||
Albumin | Forward | 5′-TGAGTTTGCAGAAGTTTCCA-3′ | 60 |
Reverse | 5′-CCTTTGCCTCAGCATAGTTT-3′ | ||
TAT | Forward | 5′-AACGATGTGGAGTTCACGG-3′ | 55 |
Reverse | 5′-GACACATCCTCAGGAGAATGG-3′ | ||
HNF1A | Forward | 5′-TACACCACTCTGGCAGCCACACT-3′ | 60 |
Reverse | 5′-CGGTGGGTACATACCTGACAGAAC-3′ | ||
CYP2B6 | Forward | 5′-CTTGACCTGCTGCTTCTTCC-3′ | 55 |
Reverse | 5′-TGCTTCCCGCCTCAGATTTCTC-3′ | ||
CYP3A4 | Forward | 5′-ATCATTGCTGTCTCCAACCTTCAC-3′ | 60 |
Reverse | 5′-TGCTTCCCGCCTCAGATTTCTC-3′ | ||
β-actin | Forward | 5′-TCCTTCTGCATCCTGTCAGCA-3′ | 58 |
Reverse | 5′-CAGGAGATGGCCACTGCCGCA-3′ |