Table 2 Human primer sequences using quantitative real-time polymerase chain reaction
Genes | Â | Primer sequences | Tm |
|---|---|---|---|
OC | Forward | 5′-CACTCCTCGCCCTATTGGC-3′ | 58 |
Reverse | 5′-CCCTCCTGCTTGGACACAAAG-3′ | ||
COL1A1 | Forward | 5′-AGACATCCCACCAATCACCT-3′ | 60 |
Reverse | 5′-CGTCATCGCACAACACCT-3′ | ||
Adipsin | Forward | 5′-GGTCACCCAAGCAACAAAGT-3′ | 60 |
Reverse | 5′-CCTCCTGCGTTCAAGTCATC-3′ | ||
PPAR-γ | Forward | 5′-TTGACCCAGAAAGCGATTCC-3′ | 60 |
Reverse | 5′-AAAGTTGGTGGGCCAGAATG-3′ | ||
PRL-1 | Forward | 5′-TACTGCTCCACCAAGAAGCC-3′ | 60 |
Reverse | 5′-AGGTTTACCCCATCCAGGTC-3′ | ||
RhoA | Forward | 5′-TGGAAAGCAGGTAGAGTTGG-3′ | 60 |
Reverse | 5′-GACTTCTGGGGTCCACTTTT-3′ | ||
ROCK1 | Forward | 5′-GAAGAAAGAGAAGCTCGAGA-3′ | 60 |
Reverse | 5′-GATCTTGTAGCTCCCGCATCTGT-3′ | ||
Alu | Forward | 5′-GGAGGCTGAGGCAGGAGAA-3′ | 55 |
Reverse | 5′-CGGAGTCTCGCTCTGTCGCCCA-3′ | ||
PGC-1α | Forward | 5′-CAGCAAAAGCCACAAAGACG-3′ | 60 |
Reverse | 5′-GGGTCAGAGGAAGAGATAAAGTTG-3′ | ||
NRF1 | Forward | 5′-GCTTCAGAATTGCCAACCAC-3′ | 60 |
Reverse | 5′-GTCATCTCACCTCCCTGTAAC-3′ | ||
mtTFA | Forward | 5′-GAACAACTACCCATATTTAAAGCTCA-3′ | 60 |
Reverse | 5′-GAATCAGGAAGTTCCCTCCA-3′ | ||
mtDNA | Forward | 5′-CCACTGTAAAGCTAACTTAGCATTAACC-3′ | 55 |
Reverse | 5′-GTGATGAGGAATAGTGTAAGGAGTATGG-3′ | ||
nuclearDNA | Forward | 5′-CCAGAAAATAAATCAGATGGTATGTAACA-3′ | 55 |
Reverse | 5′-TGGTTTAGGAGGGTTGCTTCC-3′ | ||
GAPDH | Forward | 5′-GCACCGTCAAGGCTGAGAAC-3′ | 60 |
Reverse | 5′-GTGGTGAAGACGCCAGTGGA-3′ |