Fig. 2

AAV9-miR-542-3p injection attenuated MCAO induced injury. AAV9-miR-542-3p and AAV9-miR-NC were injected into paracele of mice at 1 h after MCAO or sham operation. Brain tissues were collected after 3 days of operation. a The expression of miR-542-3p in brain tissues after AAV9 injection was tested using qRT-PCR. b TTC staining indicated the infarcted area of brain tissues. c The brain water content in brain was detected. d Neurological grading scores of mice were evaluated. e FJB staining was used to detect the degenerating neurons. Scale bar 20 μm. f H&E staining showed the structure of brain in different groups. Scale bar 20 μm. g IHC staining for VEGFA expression in brain tissues. Scale bar 50 μm. h Inflammatory factors TNFα and MCP-1 were detected using qRT-PCR. i Western blot was used to test the protein expression of TLR4, TNFα, and IL 6. Data are mean ± SD; *P < 0.05, **P < 0.01. All experiments were repeated in triplicate