Fig. 3

MiR-542-3p alleviated OGD induced glial cell damage. MiR-542-3p or miR-NC was transfected into HA1800 cells; then, cells were treated with or without OGD for 2 h. a MiR-542-3p expression in HA1800 cells was detected using qRT-PCR after transfection or OGD treatment. b Cell viability was tested by MTT. c TUNEL assay was used to calculate cell apoptotic level. Scale bar 20 μm. d ROS assay was used to determine the production of ROS in HA1800 cells. Scale bar 25 μm. e Inflammatory factors TNFα and MCP-1 were detected using qRT-PCR. f Western blot was used to test the protein expression of TLR4, TNFα, and IL 6. Data are mean ± SD; *P < 0.05, **P < 0.01. All experiments were repeated in triplicate