Fig. 5

Exosomal miR-542-3p remitted MCAO induced inflammation. MiR-542-3p was transfected into MSCs; exosomes in supernatant were isolated and injected into paracele of mice with or without MCAO. Brain tissues were collected after 3 days of operation. a MiR-542-3p expression in brain tissues was tested using qRT-PCR. b TTC staining for infarcted area of brain tissues. c The brain water content in brain was detected. d Neurological grading scores of mice were evaluated. e FJB staining was used to detect the degenerating neurons. Scale bar 20 μm. f H&E staining showed the structure of brain in different groups. Scale bar 20 μm. g IHC staining was used to detect VEGFA level brain in different groups. Scale bar 50 μm. h Inflammatory factors TNFα and MCP-1 were detected using qRT-PCR. i Western blot tested the protein expression of TLR4, TNFα, and IL 6. Data are mean ± SD; *P < 0.05, **P < 0.01. All experiments were repeated in triplicate