Fig. 4

Application of ChABC has no effect on ENSC spread/behaviour. a, b Representative images of transplanted ENSCs within the ENSC-treated (a) and ENSC+ChABC-treated (b) spinal cords. At the time of tissue harvest (16 weeks post-injury), numerous GFP+ cells were found within sagittal spinal cord sections of both the ENSC-only group (a) and the ENSC+ChABC groups (b). c–f Serial spinal cord sections spanning the extent of GFP+ cell detection were analysed for cell spread across the anterior/posterior (c), dorsal/ventral (d) and left/right planes (e), as well as for cell survival (f). No significant differences were detected in any parameter between animals treated with ENSCs+ChABC or with ENSC-only. Data are represented as mean ± s.e.m. Scale bars—a, b 500 μm