Table 4 PCR primers
Probe target | Primer sequence | Product size | Tm |
|---|---|---|---|
Gfp | F: CACATGAAGCAGCACGACTT R: TCCTTGAAGTCGATGCCCTT | 167 | 59.13 59.02 |
Gapdh | F: GTTGTGGATCTGACATGCCG R: GGTGGAAGAATGGGAGTTGC | 171 | 59.27 58.82 |
Sox10 | F: ACCTCCACAATGCTGAGCTC R: CGCCGAGGTTGGTACTTGTA | 160 | 60.04 59.76 |
Tuj1 | F: TGACGAGCATGGCATAGACC R: AATAGGTGTCCAAAGGCCCC | 192 | 59.9 59.66 |
Gls1 | F: GTGTGTTCAAAGCAACATCGTT R: ACACCCCACAAATCAGGACT | 195 | 58.57 58.85 |
nNos | F: AGGACAACGTTCCTGTGGTC R: CCGTCTCCCAGTTCTTGACC | 150 | 59.89 60.04 |
Tph1 | F: TGCGACATCAACCGAGAACA R: CGCAGAAGTCCAGGTCAGAA | 172 | 59.97 59.68 |
Chat | F: TTTGATGGCATCGTCCTGGT R: CGAGATGGCCTTGGGTTTCT | 166 | 59.67 60.04 |
Gad | F: GAGTCGTCTTGTGAGTGCCT R: GTTTGCTCCTCCCCGTTCTT | 171 | 59.68 60.25 |
S100b | F: TCAGGGAGAGAGGGTGACAA R: TCATGACAGGCTGTGGTCAC | 218 | 59.51 59.96 |