Fig. 4

MEG3 inhibits telomerase activity via long noncoding RNA TERRA. A Quantitative RT-PCR was used to detect the expression of TERRA. β-actin was used as an internal reference gene. B RNA pulldown analysis was performed using biotin-labeled TERRA probe or biotin-labeled TERC probe and anti-TERT. C RIP with anti-TERT. IgG RNA co-immunoprecipitation was used as a negative control. D (a) Super-RNA-protein complex gel migration assay (Super-EMSA) using the biotin-labeled TERRA RNA probe (Biotin-TERRA) and anti-TERT. IgG super-EMSA was used as a negative control. D (b) Quantitative analysis of gray scales of positive bands. E After cross-linking formaldehyde, RIP using anti-TERT was performed. IgG RNA co-immunoprecipitation was used as a negative control. F The telomerase activity was examined by the quantitative telomerase activity assay (TRAP)