Fig. 2

Expression of Mst1 in mBM-MSCs exposed to H₂O₂ condition. a Cell viability was measured in mBM-MSCs treated with different H₂O₂ concentrations for 12 h by CCK-8 (n = 3). b Mst1 expression in mBM-MSCs treated with different concentrations of H₂O₂ for 12 h. c, d mBM-MSCs were transfected with Ad-LacZ or Ad-sh-Mst1, and the Mst1 expression was confirmed by qPCR and Western blot analysis (n = 3). e Effects of Mst1 inhibition on the cell viability of mBM-MSCs exposed to H₂O₂ condition. f Cell adhesion of mBM-MSCs/sh-Mst1 exposed to H₂O₂ condition (n = 3). Data are expressed as mean ± SD. **p < 0.01, *p < 0.05. H₂O₂, mBM-MSCs exposed to hydrogen peroxide only; H₂O₂ + Neg, mBM-MSCs transduced with adenoviruses harboring control vectors for Ad-sh-Mst1 (Ad-LacZ) followed by exposure to H₂O₂; H₂O₂ + sh-Mst1, mBM-MSCs transduced with adenoviruses expressing sh-RNA directed against Mst1 (Ad-sh-Mst1) followed by exposure to H₂O₂; H₂O₂ + sh-Mst1 + 3-MA, mBM-MSCs/sh-Mst1, and pretreated with 3-MA followed by exposure to H₂O₂