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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Single-cell RNA sequencing of equine mesenchymal stromal cells from primary donor-matched tissue sources reveals functional heterogeneity in immune modulation and cell motility

Fig. 6

Intra-source heterogeneity of peripheral blood (PB)-derived mesenchymal stromal cells (MSCs) translates into varying chemoattractant capacity. a C-X-C motif chemokine ligand 6 (CXCL6) expression in 14 clones generated from a bulk PB-derived MSC culture, as determined by qRT-PCR. b UMAP representation of PB-derived MSC clusters. Points are colored by unsupervised clustering assignment. c CXCL6 RNA expression pattern in PB-derived MSCs. Expression values are scaled from 2nd to 98th percentile of gene expression across all plotted cells. d CXCL6 RNA expression across PB-derived MSC clusters. Dot size is proportional to number of cells with detectable expression of CXCL6. Dot color intensity indicates gene expression values scaled across plotted clusters. e Schematic of the neutrophil chemotaxis assays, with insert showing hematoxylin stained neutrophils and MSC, after neutrophil migration (i) and quantification of neutrophil migration toward the bulk PB-derived MSC population, clone 5 MSCs, and clone 6 MSCs (ii). f Schematic of the neutrophil chemotaxis assays, with insert showing hematoxylin stained neutrophils after migration (i) and quantification of neutrophil migration toward the conditioned medium (CM) from the bulk PB-derived MSC population, clone 5 MSCs, and clone 6 MSCs (ii). Significant differences are depicted by different letters, n = 3. Data are presented as the mean ± standard deviation. P < 0.05

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