Fig. 1

Classification of in vitro osteogenic differentiation potential of 17 AD-MSC lines. a Strategy used for the quantification of differentiation and AD-MSC classification: (1) Cells were differentiated in vitro into osteogenic lineage, and at three time points (days 14, 17, and 21), they were stained with Alizarin Red staining. (2) For each cell line, images of the whole well were acquired and pixels were counted and summed for the three time points (days 14, 17, and 21). (3) Interquartile distribution was applied, and it was decided that the 4th quartile was representing “good,” the 3rd and the 2nd quartile represented the “intermediate,” and the 1st quartile represented the “bad” differentiating lines. b Sum of the pixels acquired at the three time points (days 14, 17, and 21) for osteogenic differentiation of all 17 AD-MSC lines and interquartile categorization into “good,” “intermediate,” and “bad” AD-MSCs. c In vitro differentiation of one representing “good,” one “intermediate,” and one “bad” AD-MSC after 14, 17, and 21 days under osteogenic condition assessed by Alizarin Red staining. Depicted are triplicates of undifferentiated cells (control) and cells cultured under differentiation conditions