Fig. 5

ALP+/CD73+ cells are present in human fat tissue and possess enhanced osteogenic potential. a Immunofluorescence of ALP and CD73 in human fat tissue. Scale 100 μm. BF, bright field. b Alizarin Red staining and quantification of 3 healthy donors’ SVF after 21 days of osteogenic differentiation. Depicted is one triplicate of undifferentiated cells (control) and triplicates of cells cultured under osteogenic differentiation conditions. Error bars indicate the triplicates of the staining and are presented as mean ± s.d. For statistical analyses, the one-way ANOVA Dunnett’s multiple comparisons test was used to compare the ALP+/CD73+ population with the other sorted fractions within the same day: *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001. ns, not significant