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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Targeted expression profiling reveals distinct stages of early canine fibroblast reprogramming are regulated by 2-oxoglutarate hydroxylases

Fig. 7

Canine reprogramming intermediates are depleted of a subset of pluripotency-associated factors expressed in cESC. a Summarization of raw counts of primary colonies emerging in the first 4 days after passaging transduced cFFs (7–10 DPI) in medium containing FBS. b Representative phase-contrast micrograph of one serially pick-passaged primary colony on (I) 10 DPI, (II) 14 DPI, (III) 18 DPI, (IV) 22 DPI, (V) 26 DPI, and (VI) 30 DPI. c Relative transcript abundance (Log10 transformed) of SeV-derived transcripts encoding KLF, KLF-OCT4-SOX2 (KOS), and MYC. Data are presented as mean ± standard error, n = 3. Means annotated with different letters are considered significantly different by one-way analysis of variance Tukey’s honestly squared difference test, P < 0.05. d Scatterplot of Log2-scaled expression ratios for a representative cESC sample versus a representative 10 DPI AA/RA sample. e Scatterplot of Log2-scaled expression ratios for a representative cESC sample versus a representative 10 DPI DMOG/AA/RA sample. Blue dots represent genes contributing to dissimilarity in the overall positive gene-gene association. f GeneMANIA network where nodes represent top differentially expressed genes in ESC versus 10 DPI primary colonies (AA/RA and DMOG/AA/RA groups). Edges represent known genetic interactions (green) or co-expression (purple) between genes based on datasets compiled by GeneMANIA. Densely interconnected nodes are shaded gray

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