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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Allogeneic human umbilical cord Wharton’s jelly stem cells increase several-fold the expansion of human cord blood CD34+ cells both in vitro and in vivo

Fig. 6

Mitochondrial behavior of UCB CD34+ cells expanded with hWJSCs and hWJSC-CM. A Note that there were greater increases in the CD34+MitoTracker-Green staining (Mean fluorescence index; MFI) cells expanded in hWJSC-CM after 7 days compared to their numbers at day 0. B Note significantly lower mitochondrial membrane potential in CD34+CMXRos+ cells expanded with hWJSCs as compared to controls. C Note significantly increased mitochondrial superoxide staining in CD34+ cells expanded with hWJSCs as compared to controls. D Note significantly lower oxidative stress (CellRox staining) in CD34+ cells expanded with hWJSCs and hWJSC-CM as compared to controls. All values are expressed as mean ± SEM of 3 biological samples with 3 replicates for each sample. Asterisk (*): p < 0.05. E (a–d) Confocal immunohistochemistry images of Mitotracker-Green CD34+ cells interacting with CellTracker-Red hWJSCs. a Low-magnification confocal image of co-culture of the two cell types under differential interference contrast (DIC) optics. b Low-magnification confocal image of MitoGreen CD34+ mitochondria. c Low-magnification confocal image of MitoGreen CD34+ cells co-cultured with CellTracker-Red-hWJSCs. d High magnification of Mitotracker-Green CD34+ cells co-cultured with CellTracker-Red hWJSCs. Note MitoTracker-Green CD34+ mitochondria lying inside the cytoplasm of CellTracker-Red hWJSCs (yellow dots)

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