Fig. 7

Glycolysis (HIF-1a and HK-1) and oxidative phosphorylation (ATP6VIH and NDUFA10) gene expression of UCB CD34+ cells expanded with hWJSCs and hWJSC-CM. a Note significantly greater HIF-1a and b HK-1 gene expression in CD34+ cells expanded with hWJSCs as compared to controls. c Note significantly lower ATP6VIH and d NDUFA10 gene expression in cells expanded with hWJSC-CM and hWJSCs as compared to controls. All values are expressed as mean ± SEM of 3 biological samples with 3 replicates for each sample. *p < 0.05. In vivo transplantation of hWJSC-expanded CD34+ cells into NSG mice. e Note significantly greater CD45+ chimerism in the bone marrow of primary NSG mice at 6 weeks post-transplantation with CD34+ cells expanded with hWJSCs as compared to uncultured UCB CD34+ cells. f Note significantly greater CD45+ chimerism in the bone marrow of secondary NSG mice at 6 weeks post-transplantation with cells isolated from bone marrow of primary NSG mice and expanded with hWJSCs as compared to uncultured UCB CD34+ cells. All values are expressed as mean ± SEM of 4 biological samples. *p < 0.05