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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Human multipotent mesenchymal stromal cells cytokine priming promotes RAB27B-regulated secretion of small extracellular vesicles with immunomodulatory cargo

Fig. 3

Characterization of cytokine-primed MSC-sEVs. a Nanoparticle tracking analysis (NTA) of primed MSC-sEVs. b NTA of MSC-sEV flotation densities following fractionation with iodixanol density gradient. c Transmission electron microscopy of negative stained MSC-sEVs. Scale bar represents 100 nm. d Western blot analysis of MSC cell extracts (CELL), conditioned media (CM), and small exosome-like EVs (sEVs) for EV-enriched proteins (CD81, ALIX) and major contaminant protein derived from subcellular organelles such as endoplasmic reticulum (CANX, calnexin)

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Stem Cell Research & Therapy

ISSN: 1757-6512