Fig. 4

sEVs from cytokine-primed MSCs contain immunomodulatory proteins. a Unique and common proteins in sEVs collected from resting and primed MSC CM (n = 3 per group). The graph includes proteins identified in at least two samples per group. b, c Gene ontology analyses of the 161 proteins shared between resting and primed MSC-sEVs. Cellular components are shown on the left panel (b) and biological processes on the right panel (c). Proteins associated with ExoCarta’s top EV 100 proteins (http://exocarta.org), and MSC markers are listed in the boxes below (gray box lists extracellular exosome/sEV-enriched proteins and dotted line box MSC markers). d, e Cellular components (d, left panel) and biological processes (e, left panel) associated with the 183 proteins uniquely present in primed MSC-sEVs (Gene Ontology analyses). Gray box below panel (d) lists extracellular exosome/sEV-enriched proteins and gray box below panel (e) lists proteins in the “inflammatory response” category. f, g Western blot analysis of TSG-6 (TNFAIP6) and A20 (TNFAIP3) in resting and primed MSC-sEVs (n = 6). Wilcoxon signed-rank test, mean ± SD are reported, (*) represents p ≤ 0.05