Fig. 3

Effects of GSK-J4 on Twist-1^del/+ viability and proliferation. a Representative cell densities of Twist-1^del/+ calvarial cells are shown following treatment with low, medium, and high doses of GSK-J4 for 1 week, scale bar = 100 μm at ×50 magnification. b Proliferation rates were measured by BrdU incorporation for Twist-1^del/+ calvarial cells following GSK-J4 treatment with a range of concentrations (0.1 μM–10 μM or 0.1% DMSO vehicle control) for 1 week. Data represent mean ± S.E., one-way ANOVA with Tukey’s multiple comparisons, n = 3 Twist-1^del/+ mice. c Flow cytometric analysis of Annexin V/7AAD staining in Twist-1^del/+ calvarial cells in the presence of GSK-J4 (0.1 μM–10 μM or 0.1% DMSO vehicle control) for 1 week. Representative histograms depicting early apoptotic cells (Annexin V⁺), necrotic cells (7AAD⁺), and late apoptotic cells (Annexin V⁺/7AAD⁺). d Quantitation of percentage of Annexin V/7AAD stained Twist-1^del/+ calvarial cells by flow cytometric analysis in the presence of GSK-J4 (0.1 μM–10 μM or 0.1% DMSO vehicle control) for 1 week. Data represent mean ± S.E., *p < 0.05, one-way ANOVA with Tukey’s multiple comparisons, n = 3 Twist-1^del/+ mice