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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis

Fig. 2

Role of hypoxia- or normoxia-preconditioned ADSCs in proliferation and apoptosis of HUVECs. a Proliferation of HUVECs influenced by secreted fractions from hypoxia- or normoxia-preconditioned ADSCs (H or N group) was evaluated with Cell Counting Kit-8 (CCK8) assay at 2, 12, 24, and 48 h after cell seeding. n = 6/group. 2, 12, 24, 48 h, *p < 0.05. b The outcome of colony formation assays formed by HUVECs influenced by secreted fractions from hypoxia- or normoxia-preconditioned ADSCs (H or N group). c Quantification of colonies formed from HUVECs. *p < 0.05. d, e Cell cycle assessment was tested by flow cytometry. HUVECs at S+G2/M phase was significantly increased after affected by secreted fractions from hypoxia-preconditioned ADSCs. *p < 0.05. f, g Cell apoptosis was determined by flow cytometry. Hypoxia-preconditioned ADSCs could significantly decrease the apoptotic percentage of HUVECs. *p < 0.05

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