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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis

Fig. 3

Role of hypoxia- or normoxia-preconditioned ADSCs in migration and angiogenesis of HUVECs. a, b Representative photos of scratch on HUVECs at 0 h and 24 h after treated by secreted fractions from hypoxia- or normoxia-preconditioned ADSCs. The migration of HUVECs was significantly increased by secreted fractions from hypoxia-preconditioned ADSCs. *p < 0.05. c, d Representative photos of transwell migration assay of HUVECs after treated by secreted fractions from hypoxia- or normoxia-preconditioned ADSCs. Hypoxia-preconditioned ADSCs increased migrated HUVECs. *p < 0.05. e In vitro tube formation of HUVECs at 10 h in different groups. f–j The quantification of branches, junctions, nodes, total length, and total branching length was listed and measured by Image-Pro Plus 6.0 (IPP). *p < 0.05

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