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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis

Fig. 4

Role of hypoxia-preconditioned ADSCs in glycolysis of HUVECs. a VEGF secreted by hypoxia- or normoxia-preconditioned ADSCs determined by ELISA. *p < 0.05. b, c The protein expression of VEGFR2, Hif-1α, and HK2 determined by WB. *p < 0.05. d The transcription level of VEGFR2, Hif-1α, and HK2 determined by RT-qPCR. *p < 0.05. e Detection of VEGF after ADSCs were transfected by siRNA. *p < 0.05. f, g The protein expression of VEGFR2, Hif-1α, and HK2 after ADSCs transfected by siRNA were determined by WB. *p < 0.05. h The transcription level of VEGFR2, Hif-1α, and HK2 after ADSCs transfected by siRNA was determined by RT-qPCR. *p < 0.05. in The angiogenic activity of hypoxia- or normoxia-preconditioned ADSCs was assessed by tube formation assay. *p < 0.05

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