Fig. 1

MSC characterization and the schematic representation of the experimental procedures. a MSCs isolated from the bone marrow of WT mice were first evaluated for the expression of mouse MSC characterization markers. Cells were gated on total MSCs for evaluation of CD44 marker. For the rest of the markers, cells were first gated on CD44⁺ cells. Red histograms depict the isotype controls, and blue histograms depict the expression of each desired marker. Thereafter, MSCs were co-cultured with b WT-T cells and c TNFR2 KO-T cells for further experimental procedures. d These FACS representatives demonstrate the depletion of CD25⁺ subpopulation from initial T cells