Fig. 3
From: Regulatory T cell induction by mesenchymal stem cells depends on the expression of TNFR2 by T cells
TNFR2 expression by T cells is directly associated with the secretion of anti-inflammatory cytokines and their immunosuppressive effect. a The secretion of IL-10 and TGFβ anti-inflammatory cytokines was assessed by the ELISA method. Total T cells collected after co-culturing with WT-MSCs were further activated or not with anti-CD3/CD28 beads for a duration of 24 h. Furthermore, Treg immunosuppressive effect was measured by performing an MLR test. Briefly, 104 MSC-exp T cells were added to 105 freshly isolated CFSE-labeled responder T cells. Responder cells were activated using anti-CD3/CD28 beads. After 3 days, the proliferation capacity of b CD4+ and c CD8+ T cell subsets was analyzed by flow cytometry (n = 4). FACS histograms demonstrate flow cytometry representatives of the proliferation assay