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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: circAKT3 positively regulates osteogenic differentiation of human dental pulp stromal cells via miR-206/CX43 axis

Fig. 3

Knockdown of circAKT3 inhibited osteogenic differentiation of hDPSCs. a Sanger sequencing proved the circular property of circAKT3. The orange arrow shows the back-splicing site. b Transfection of sh1-circAKT3 or sh2-circAKT3 achieved knockdown of circAKT3 in hDPSCs as qRT-PCR suggested. c ALP and ARS staining of hDPSCs transfected by sh-NC and sh1-circAKT3. d Quantification of ALP and ARS staining. e Relative mRNA expression of ALP, RUNX2, and OCN was detected by qRT-PCR after osteogenic induction for 7 days. f Protein expression of RUNX2, OCN, ALP, and GAPDH after osteogenic induction detected by Western blot analysis. g Gray values of bands of RUNX2, OCN, ALP, and GAPDH *p < 0.05 and **p < 0.01 compared to the group of sh-NC. Three biological replicates were collected in each group

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