Fig. 6

CGP promotes the proliferation of NSCs and activates the cAMP–CREB signaling pathway in vitro. a The confocal microscopic images of BrdU (red)/DAPI (blue) stained NSCs after incubation with vehicle or CGP for 24 h (scale bar 100 μm). b Histograms showing the quantitation of BrdU-positive cells (the data are expressed as the mean ± SD, n = 5 samples per condition). **P < 0.01 compared with the vehicle group. c The histogram shows the cAMP levels detected by the ELISA assay. d–f (upper) Representative bands of cAMP, PKA, pCREB, and CREB. d–f (lower) Expression of PKA, pCREB, and CREB in each group determined by Western blot. Western blot quantitation was performed using densitometric analysis (the data are expressed as the mean ± SD, n = 5 samples per condition). **P < 0.01 compared with the vehicle group (unpaired Student’s t test with Kolmogorov–Smirnov test)