Fig. 3

Effect of ADSCC-CM on HKFs. a The total protein detection of CM, CM5, and CM10. b The proliferation of HKFs was detected after CM, CM5, and CM10 intervention for 0 h, 24 h, and 48 h, respectively. DM was set as control. c, d HKFs were treated with CM, CM5, and CM10 for 48 h (n = 3). Apoptosis was detected. e HKFs were treated with CM, CM5, and CM10 for 48 h (n = 3). The expression of α-SMA in HKFs was detected by flow cytometry. f Immunocytochemical of fibroblasts: α-SMA was labelled with Alexa Fluor®488 (green), and the nucleus was stained with DAPI (blue). DAPI, 4′,6-diamidino-2-phenylindole