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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human umbilical cord mesenchymal stem cell-derived exosomal miR-27b attenuates subretinal fibrosis via suppressing epithelial–mesenchymal transition by targeting HOXC6

Fig. 2

Identification of hucMSC-derived exosomes (hucMSC-Exo). a The hucMSC-Exo were isolated from the conditioned medium using serial centrifugation. b Transmission electron microscopic images of typical hucMSC-Exo. Scale bar = 100 nm. c Size distribution of the hucMSC-Exo was determined using a nanoparticle tracking analysis. hucMSC-Exo had an original concentration of 8.8e+9 particles/mL, a mean size of 159.0 nm, and a peak size of 128.6 nm. d Positive expression of markers CD9, TSG101, CD63, and HSP70 was mainly detected in hucMSC-Exo using Western blot analysis. e Fluorescence result of ARPE19 cells after being co-cultured with hucMSC-Exo labeled with PKH67, observed under a confocal microscope (scale bar = 25 μm)

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Stem Cell Research & Therapy

ISSN: 1757-6512