Fig. 8
From: CCR2 improves homing and engraftment of adipose-derived stem cells in dystrophic mice
CCR2-ADSC transplantation further improves muscle pathology in mdx mice. Assessment of muscle pathology using haematoxylin and eosin (H&E) and Sirius Red staining. The decreased percentage of myofibres with central nuclei correlated with the decreased severity of muscle injury in mdx mice. Moreover, muscle fibrosis was assessed using Sirius Red staining, which revealed collagen expression. The decreased collagen expression levels correlated with the decreased severity of muscle fibrosis in mdx mice. a H&E-stained images and b the percentages of myofibres with central nuclei in the tibialis anterior (TA) of C57, mdx + PBS, mdx + VEH-ADSC, and mdx + CCR2-ADSC mice at 1-month, 6-month, and 1-year post-transplantation of ADSCs. c H&E-stained images and d the percentages of myofibres with central nuclei in the quadriceps of C57, mdx + PBS, mdx + VEH-ADSC, and mdx + CCR2-ADSC at 1-month, 6-month, and 1-year post-transplantation of ADSCs. Fibrosis in the TA (e) and quadriceps (f) of C57, mdx + PBS, mdx + VEH-ADSC, and mdx + CCR2-ADSC mice at 1-month, 6-month, and 1-year post-transplantation of ADSCs. Scale bar = 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001