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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Myocardial repair of bioengineered cardiac patches with decellularized placental scaffold and human-induced pluripotent stem cells in a rat model of myocardial infarction

Fig. 5

BCP reduced infarct size via improvement of hiPSC-CM survival after transplantation. a Representative Masson’s trichrome staining of heart sections 4 weeks after DP, hiPSC-CM, and BCP transplantation. b Representative immunofluorescent staining of human nuclei antigen (HNA; red), cardiac troponin T (cTnT; green), and DAPI (blue) at the peri-infarct zone after DP, hiPSC-CM, and BCP transplantation. c Infarct size as measured by the percentage area with collagen deposition by trichrome staining indicating that hiPSC-CMs and BCP reduced the infarct size 4 weeks after of transplantation. d The quantitative data of HNA and cTNT-positive cells indicate that hiPSC-CMs in the BCP had an improved survival rate compared with hiPSC-CMs only; Student’s t test was used to compare MI + CM and MI + BCP groups (n = 9; P < 0.05). e Co-immunofluorescent staining of a-smooth muscle actin (a-SMA) and cTnT in the peri-infarct zone from after MI alone and with DP, hiPSC-CM, and BCP transplantation showed increased neovascularization following BCP transplantation. Data are shown as mean ± SEM. n = 9, Four-group comparisons were performed using one-way ANOVA followed by Tukey’s post hoc test. *P < 0.05 compared with MI group. f Representative immunoblot (left panel) and protein expression (right panel) of angiogenin, angiopoitin-2, and VEGF to demonstrate that VEGF, but not angiogenin or angiopoietin-2, were significantly increased in the peri-infarct area after transplantation of hiPSC-CMs and BCP compared with the MI group. Four-group comparisons were performed using one-way ANOVA followed by Tukey’s post hoc test (n = 3; *P < 0.05 compare to MI group)

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