Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: microRNA-27b shuttled by mesenchymal stem cell-derived exosomes prevents sepsis by targeting JMJD3 and downregulating NF-κB signaling pathway

Fig. 1

Exosomal miR-27b in mouse BMMSCs inhibits CLP-induced sepsis. a Isolation and identification of mouse MSCs, bright field (BF), CD29, Sca-1 and CD34 (green), DAPI (blue). b TEM micrographs of mouse MSC-derived exosomes. c Size distribution and concentration of particles as quantified by NTA. d The expression of miR-27b in mouse MSC-derived exosomes analyzed by RT-qPCR. *p < 0.05 vs. exosomes derived from mouse fibroblasts L929. e Measurements of a 7-day survival of mice by Kaplan-Meier method. n = 10 for mice following each treatment. f The production of TNF-α, IL-1β, IL-10, and IL-6 in serum of CLP-induced septic mice injected with MSC-EXO, MSC-miR-27b-inhibitor-EXO, and MSC-NC-inhibitor-EXO examined by ELISA. g Detection of ALT, AST, and SCr levels in the serum of mice. h Bacterial colonies in peritoneal fluid and serum of mice. *p < 0.01 vs. CLP-induced mice; #p < 0.001 vs. mice with sham operation; &p < 0.01 vs. CLP-induced septic mice treated with MSC-miR-27b-inhibitor-EXO. n = 10 for mice following each treatment. Quantitative data were presented as mean ± standard deviation. Unpaired data in compliance with normal distribution and equal variance between two groups were compared using unpaired t test. Comparisons among multiple groups were analyzed by one-way ANOVA with Tukey’s post hoc test. p < 0.05 indicated significant difference

Back to article page