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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: microRNA-27b shuttled by mesenchymal stem cell-derived exosomes prevents sepsis by targeting JMJD3 and downregulating NF-κB signaling pathway

Fig. 2

miR-27b prevents LPS-mediated BMDM inflammation through suppression of JMJD3. a Target genes of miR-27b predicted by the Starbase website. b, c Binding sites (b) of miR-27b to JMJD3 and verification (c) by dual-luciferase reporter gene assay. *p < 0.01 vs. BMDMs transfected with NC-mimic. d Images of BMDMs uptaking exosomes visualized under a LSCM. e The expression of miR-27b in BMDMs treated with LPS or MSC-NC-mimic-EXO and MSC-miR-27b-mimic-EXO analyzed by RT-qPCR. f The expression of JMJD3 and H3K27me3 in BMDMs treated with LPS or MSC-NC-mimic-EXO and MSC-miR-27b-mimic-EXO analyzed by western blot. In panel 2 e, f, *p < 0.05 vs. PBS-treated BMDMs; #p < 0.05 vs. LPS-treated BMDMs; &p < 0.05 vs. BMDMs treated with MSC-NC-mimic-EXO. The experiment was repeated 3 times independently. Quantitative data were presented as mean ± standard deviation. Unpaired data in compliance with normal distribution and equal variance between two groups were compared using unpaired t test. Comparisons among multiple groups were analyzed by one-way ANOVA with Tukey’s post hoc test. p < 0.05 indicated significant difference

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