Fig. 4

MSC-derived exosomal miR-27b inhibits JMJD3 and NF-κB/p65 axis to restrain LPS-induced pro-inflammatory response. a–c The recruitment of JMJD3, p65, H3K27me3, and H3K27me1 in the promoter region of pro-inflammatory cytokines TNF-α (a), IL-1β (b), and IL-6 (c) in LPS-treated BMDMs transfected with MSC-miR-27b-inhibitor-EXO analyzed by CHIP-qPCR. *p < 0.05 vs. LPS-treated BMDMs transfected with MSC-NC-inhibitor-EXO. d–f The expression of pro-inflammatory cytokines TNF-α (d), IL-1β (e), and IL-6 (f) in BMDMs after overexpression of JMJD3 or p65. *p < 0.05 vs. PBS-treated BMDMs. The experiment was repeated 3 times independently. Quantitative data were presented as mean ± standard deviation. Unpaired data in compliance with normal distribution and equal variance between two groups were compared using unpaired t test. Comparisons among multiple groups were analyzed by one-way ANOVA with Tukey’s post hoc test. p < 0.05 indicated significant difference