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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Potential of resveratrol in enrichment of neural progenitor-like cell induction of human stem cells from apical papilla

Fig. 3

Optimal condition of resveratrol pre-treatment. a The expression of NES of resveratrol pre-treatment for 12 h ranging from 0 to 50 μM. The NES expression was significantly increased at 10 μM and dropped at 50 μM. b The NES expression of resveratrol pre-treatment at 10 μM for 1, 6, 12, and 24 h. The highest expression of NES was observed at 12 h and determined as an optimal condition. c Neural progenitor gene makers of resveratrol at 10 μM for 12 h condition were validated with SOX1 and PAX6. Data were expressed as the mean ± SEM; n = 3, ***p < 0.001. d The cell morphology visualized by β-III tubulin staining of RSV-hSCAPs during 0–50 μM of resveratrol for 12 h. e The β-III tubulin profiling of RSV-hSCAPs at 10 μM for 1, 6, 12, and 24 h. f Nestin, β-III tubulin, Ki67, and NF immunostaining. These outcomes have strongly demonstrated that pre-treatment of resveratrol effectively induces neural progenitor gene marker expression but insufficiently triggers morphological change of the hSCAPs. Scale bars: d, e = 50 μm and f = 100 μm

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