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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Mesenchymal stem cell-secreted prostaglandin E2 ameliorates acute liver failure via attenuation of cell death and regulation of macrophage polarization

Fig. 3

MSC-derived PGE2 inhibits NLPR3 inflammasome activation in liver macrophages. a Protein levels of NLRP3, Caspase 1 p20, and mature-IL-1β in each group. b Serum levels of IL-1β in each group (n = 6). c Measurement of caspase-1 enzymatic activity in the liver of each group (n = 4). d Representative immunofluorescence staining of co-localization of NLRP3 with F4/80 in the liver of each group. Blue: DAPI; green: NLRP3; red: F4/80. White arrows in the Merge images indicated co-localization. e Protein levels of NLRP3 inflammasome pathway in hepatocyte cell line AML12 and BMDM treated with 1 μg/ml LPS for 24 h. f The levels of IL-1β in supernatants of hepatocyte cell line AML12 and BMDM treated with 1 μg/ml LPS for 24 h (n = 3) (*p < 0.05, **p < 0.01, ***p < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512