Fig. 4

Glucocorticoid regulated the colocalization of FPR and CXCR4 of bone marrow-derived mesenchymal stem cells. a Colocalization of FPR and CXCR4 in MSCs after double immunofluorescence staining, deconvolution, and spotted treatment. b 2D histogram of Pearson’s coefficient in colocalized volume in MSCs. c Scatterplot of the vesicle relative position of FPR and CXCR4 after vantage treatment in MSCs. Pearson’s coefficient in colocalized volume was significantly decreased in MSCs after 75 ng/ml corticosterone treatment. When pretreated with RU486 (10 μm), Pearson’s coefficient in the colocalized volume of corticosterone-treated MSCs was obviously reversed compared to the 75 ng/ml GC group. Meanwhile, it was also reversed when pretreated with Cyc H (1 μm), a specific FPR antagonist. **P < 0.01 vs. the 0 ng/ml GC group, ^#P < 0.05 vs. the 75 ng/ml group. Data are one of three representative observations