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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Improvement of the therapeutic capacity of insulin-producing cells trans-differentiated from human liver cells using engineered cell sheet

Fig. 1

Schematic description of the experimental procedure. a Preparation of insulin-producing cells from liver cells using transcription factors, process for the injection of insulin-producing cells (IPCs) through the portal vein, formation of IPC sheets on the temperature-responsive dish, and transplantation of IPC sheets onto the liver surface. b Procedure for harvesting of IPC sheets from the temperature-responsive dish. IPC or liver cells were cultured for 2 days and allowed to develop cell-cell interactions. At this point, the temperature was lowered to 25 °C for 10 min, and then, the cell sheet was allowed to float in the dish

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