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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Improvement of the therapeutic capacity of insulin-producing cells trans-differentiated from human liver cells using engineered cell sheet

Fig. 4

Comparison of mRNA expression and insulin production between insulin-producing cell (IPC) sheets and IPCs cultured on a conventional cell culture plate. a Real-time qPCR analysis of gene expression in liver cells and IPCs. IPCs were confirmed to express endocrine hormones, including insulin, glucagon, somatostatin, and pancreatic-specific genes, including PDX1, NGN3, NEUROD1, NKX6.1, and glucose transporter 2, compared to those in liver cells. The results normalized to GAPDH gene expression for the same cDNA sample are represented as the relative levels of the mean ± SD; P < 0.05 indicates a significant difference. b Insulin and c C-peptide contents and d insulin secretion in IPCs and IPC sheets after 5 days of culture. e Glucose stimulated insulin secretion index. The stimulation index was calculated as a ratio between the insulin secreted at high and low glucose media

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