Table 1 Primer sequences for RT-qPCR
Target | Primer sequences |
|---|---|
α-SMA (hsa) | F 5-TATCCCCGGGACTAAGACGGG-3 |
R 5-CAGAGCCCAGAGCCATTGTC-3 | |
α-SMA (mmu) | F 5-CGAGCGTGAGATTGTCCGT-3 |
R 5-CCCCTGACAGGACGTTGTT-3 | |
Collagen I (hsa) | F 5-CGAAGACATCCCACCAATCAC-3 |
R 5-CAGATCACGTCATCGCACAAC-3 | |
Collagen I (mmu) | F 5-ACGCATGGCCAAGAAGACAT-3 |
R 5-TTGTGGCAGATACAGATCAAGCA-3 | |
SOX4 (hsa) | F 5-AACCCCAGCTCAAACTTTGAGA-3 |
R 5-GAACCCCAGCTCAAACTTTGAG-3 | |
SOX4 (mmu) | F 5-AACCCCAGCTCAAACTTTGAGA-3 |
R 5-GAACCCCAGCTCAAACTTTGAG-3 | |
DKK1 (hsa) | F 5-CGCCGAAAACGCTGCAT-3 |
R 5-TTTCCTCAATTTCTCCTCGGAA-3 | |
DKK1 (mmu) | F 5-GGGAGTTCTCTATGAGGGCG-3 |
R 5-AAGGGTAGGGCTGGTAGTTG-3 | |
U6 (hsa and mmu) | F 5-AGGCGTCACTTTGACTTGG-3 |
β-actin (hsa) | F 5-AGG GGCCGGACTCGTCATACT-3 |
R 5-GGCGGCAC CACCATGTACCCT-3 | |
β-actin (mmu) | F 5-GTGACGTTGACATCCGTAAAGA-3 |
R 5-GCCGGACTCATCGTACTCC-3 | |
miR-186 | F 5-GGGCAAAGAATTCTCCTTT-3 |
R 5-GTGCAGGGTCCGAGGT-3 |