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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Intramuscular injection of Botox causes tendon atrophy by induction of senescence of tendon-derived stem cells

Fig. 6

a Gross view of in vitro static culture of tendon construct using isolated TDSC from the control and Botox group, and the contraction rate was measured by the width of the construct (indicated by white arrowhead). The width of Botox showed faster contraction rate (n = 3; mean ± SD; *p < 0.05, **p < 0.005). b Schematics of uniaxial mechanical loading system and H&E staining of post-loading tendon construct. The direction of uniaxial mechanical stretching is indicated by the white arrows. c mRNA expression of tendon specific markers (TMND, MKX and Scleraxis) from both the Botox and control groups. Individual mRNA expression levels were first normalised to internal control (36B4) and then compared to mRNA expression level from the control group (n = 3; mean ± SD; **p < 0.005, ***p < 0.001). d Western blot of TMND in both group and quantification and were normalised to beta-actin. Results were quantified by greyscale density (n = 3; mean ± SD; **p < 0.005, ***p < 0.001)

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