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Table 1 Different treatments and experimental design

From: PDGF-BB and IL-4 co-overexpression is a potential strategy to enhance mesenchymal stem cell-based bone regeneration

Group Genetically modified method Treatment
MSCs N/A Culture medium/osteogenic medium
MSCs-V Infected with empty control lentivirus vector Culture medium/osteogenic medium
MSCs-IL-4 Infected with mIL-4 secreting lentivirus vector Culture medium/osteogenic medium
MSCs-PDGF Infected with hPDGF-BB secreting lentivirus vector Culture medium/osteogenic medium
MSCs-IL4-PDGF Co-infected with both mIL-4 and hPDGF-BB lentivirus vectors, half of the dosage used in single infection respectively Culture medium/osteogenic medium
MSCs+(PDGF) N/A Culture medium/osteogenic medium + 0.5 ng/ml recombinant hPDGF-BB protein
MSCs-V+(PDGF) Infected with control lentivirus vector Culture medium/osteogenic medium + 0.5 ng/ml recombinant hPDGF-BB protein
MSCs-IL-4+(PDGF) Infected with mIL-4 secreting lentivirus vector Culture medium/osteogenic medium + 0.5 ng/ml recombinant hPDGF-BB protein