Table 1 Primers used for qPCR amplification
Gene | Forward sequence (5′-3′) | Reverse sequence (5′-3′) |
|---|---|---|
Lgr5 | ACCCGCCAGTCTCCTACATC | GCATCTAGGCGCAGGGATTG |
OLFM4 | TGGCCCTTGGAAGCTGTAGT | ACCTCCTTGGCCATAGCGAA |
Bmi1 | GCCACTACCATAATAGAATGTCT | TTGTGAACCTGGACATCACAAA |
Axin2 | GCAAACTTTCGCCAACCGTG | CTCTGGAGCTGTTTCTTACTGCCC |
Muc2 | GCTGACGAGTGGTTGGTGAATG | GATGAGGTGGCAGACAGGAGAC |
Alpi | GGCTACACACTTAGGGGGACCTCCA | AGCTTCGGTGACATTGGGCCGGTT |
Chga | AGGTGATGAAGTGCGTCCTG | GGTGTCGCAGGATAGAGAGGA |
Occludin | GGACCCTGACCACTATGAAACAGACTAC | ATAGGTGGATATTCCCTGACCCAGTC |
IL-1β | GCAACTGTTCCTGAACTCAAC | ATCTTTTGGGGTCCGTCAACT |
TNF-α | GGAACACGTCGTGGGATAATG | GGCAGACTTTGGATGCTTCTT |
IL-6 | TCCATCCAGTTGCCTTCTTG | GGTCTGTTGGGAGTGGTATC |
MCP-1 | CTCACCTGCTGCTACTCATTC | GCTTGAGGTGGTTGTGGAAAA |
Foxp3 | ACAACCTGAGCCTGCACAAGTT | GCCCACCTTTTCTTGGTTTTG |
IL-10 | AGGGCCCTTTGCTATGGTGT | TGGCCACAGTTTTCAGGGAT |
IDO1 | ATTGGTGGAAATCGCAGCTTC | ACAAAGTCACGCATCCTCTTAAA |
COX2 | CCAGCACTTCACCCATCAGTT | ACCCAGGTCCTCGCTTATGA |
PTEGS3 | ATCACATGGGTGGTGATGAGGA | AGGCGATGACAACAGCCCTTAC |
β-actin | AGCTGCGTTTTACACCCTTT | AAGCCATGCCAATGTTGTCT |