Fig. 2
From: Exosomal microRNA-22-3p alleviates cerebral ischemic injury by modulating KDM6B/BMP2/BMF axis
ASC-EVs alleviate cerebral ischemic injury through miR-22-3p. a RT-qPCR analysis of miR-22-3p expression in neurons. b RT-qPCR analysis of miR-22-3p expression in cerebral tissues. c Representative microscopic images of FITC-miR-22-3p mimics in neurons (× 400). d RT-qPCR analysis of miR-22-3p expression in neurons upon addition of RNase A in CM. e RT-qPCR analysis of miR-22-3p expression in neurons upon treatment of actinomycin D and ASC-EVs. f RT-qPCR analysis of miR-22-3p expression in ASCs upon miR-22-3p inhibitor. g RT-qPCR analysis of miR-22-3p expression in neurons incubated with the EVs from ASCs transfected with miR-22-3p inhibitor. h CCK-8 assay detection of neuron viability upon treatment of miR-22-3p inhibitor. i Flow cytometry detection of apoptosis upon treatment of miR-22-3p inhibitor. *p < 0.05, **p < 0.01, and ***p < 0.001. Measurement data were presented as mean ± standard deviation. The data among multiple groups were analyzed by ANOVA