Fig. 3
From: Exosomal microRNA-22-3p alleviates cerebral ischemic injury by modulating KDM6B/BMP2/BMF axis
KDM6B is a target gene of miR-22-3p derived from ASC-EVs. a Specific binding site of KDM6B and miR-22-3p predicted by an online website. b Dual-luciferase reporter gene assay of KDM6B and miR-22-3p. c RT-qPCR analysis of miR-22-3p expression in neurons upon transfection of miR-22-3p mimic or inhibitor. d RT-qPCR analysis of KDM6B expression in neurons upon transfection of miR-22-3p mimic or inhibitor. e Western blot analysis of KDM6B expression in neurons upon transfection of miR-22-3p mimic or inhibitor. f RT-qPCR analysis of miR-22-3p expression in neurons upon treatment of ASC-EVs. g RT-qPCR of KDM6B expression in neurons upon treatment of ASC-EVs. h Western blot analysis of KDM6B expression in neurons upon treatment of ASC-EVs. *p < 0.05, **p < 0.01, and ***p < 0.001. Measurement data were presented as mean ± standard deviation. The data among multiple groups were analyzed by ANOVA