Fig. 5
From: Exosomal microRNA-22-3p alleviates cerebral ischemic injury by modulating KDM6B/BMP2/BMF axis
Overexpressed KDM6B limits the protective effect of ASC-EVs on neurons. a RT-qPCR analysis of miR-22-3p expression in neurons upon treatment of ASC-EVs, OE-KDM6B, or PBS. b RT-qPCR analysis of KDM6B expression in neurons upon treatment of ASC-EVs, OE-KDM6B, or PBS. c Western blot analysis of KDM6B expression in neurons upon treatment of ASC-EVs, OE-KDM6B, or PBS. d CCK-8 assay detection of cell viability of neurons upon treatment of ASC-EVs, OE-KDM6B, OE-NC, or PBS. e Flow cytometry detection of apoptosis of neurons upon treatment of ASC-EVs, OE-KDM6B, OE-NC, or PBS. f Western blot analysis of cleaved caspase 3, caspase 3, Bcl-2, and Bax expression in neurons upon treatment of ASC-EVs, OE-KDM6B, OE-NC, or PBS. *p < 0.05, **p < 0.01, and ***p < 0.001. Measurement data were presented as mean ± standard deviation. The data among multiple groups were analyzed by ANOVA