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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Exosomal microRNA-22-3p alleviates cerebral ischemic injury by modulating KDM6B/BMP2/BMF axis

Fig. 7

ASC-EV-miR-22-3p suppresses KDM6B-mediated BMP2/BMF axis and alleviates ischemic injury. a RT-qPCR analysis determining the efficiency of si-BMF (siRNA1, siRNA2, and siRNA3). b Western blot analysis determining the efficiency of si-BMF (siRNA1, siRNA2, and siRNA3). *p < 0.05 vs. si-NC, **p < 0.01, and ***p < 0.001. c RT-qPCR analysis of miR-22-3p, KDM6B, BMP2, and BMF mRNA expression in neurons upon treatment of si-BMF and EVs. ***p < 0.001 vs. EV-inhibitor NC + si-NC and ###p < 0.001 vs. EV-miR-22-3p inhibitor + si-NC. d Western blot analysis of miR-22-3p, KDM6B, BMP2, and BMF expression in neurons upon treatment of si-BMF and EVs. e CCK-8 assay detection of viability upon treatment of EV-inhibitor NC, siBMF, EV-miR-22-3p inhibitor, and si-NC. f Flow cytometry detection of apoptosis in neurons upon treatment of EV-inhibitor NC, si-BMF, EV-miR-22-3p inhibitor, and si-NC. g Cerebral infraction volume with TCC staining. h NeuN immunofluorescence of positive NeuN cells upon treatment of ASC-EVs, PBS, and sham operation. i TUNEL staining of neurons apoptosis upon treatment of ASC-EVs, PBS, and sham operation. From f to i, *vs. EV-miR-22-3p inhibitor + NC: *p < 0.05, **p < 0.01, and ***p < 0.001; #vs. EV-miR-22-3p inhibitor + si-NC: #p < 0.05, ##p < 0.01, and ###p < 0.001. Measurement data were presented as mean ± standard deviation. The data between two groups were analyzed by independent t test. The data among multiple groups were analyzed by ANOVA

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