Fig. 3

Bleomycin shows little cytotoxicity in AF cells in vitro. a, b Cell Count Kit-8 test of AF cells and NP cells were stimulated with bleomycin in different concentrations of 0, 1, 5, and 10 μg/ml and different time period ranged from 0 to 96 h. c AF cells and NP cells were treated with bleomycin in a concentration of 0, 5, and 10 μg/ml and stained with Annexin V and PI, then subjected to flow cytometric analysis. d, e Quantification of early and late apoptotic cells rate using Graphpad8.0 by ordinary one-way ANOVA test. f AF cells and NP cells were treated with bleomycin in a concentration of 5 and 10 μg/ml then stained with Comet assay kit. g The degree of DNA damage was measured by comet tail distance using Graphpad8.0 by ordinary one-way ANOVA test. All data are presented as mean ± sd. from three experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001