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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Bleomycin induces fibrotic transformation of bone marrow stromal cells to treat height loss of intervertebral disc through the TGFβR1/Smad2/3 pathway

Fig. 5

Bleomycin activated the TGF-beta signaling pathway in AF cells. ad Western blot analysis of phospho-Smad2, phospho-Smad3, Smad2, and Smad3 in AF cells with bleomycin of 0, 5, and 10 μg/ml pretreated with LY346947 or not and quantification of grayscale value. e Western blot analysis of TGFβ, TGFβR1, FSP1, and Col1a1 in AF cells uses β-actin as a reference. f, g Western blot analysis of phospho-Smad2, phospho-Smad3, Smad2, and Smad3 in AF cells with or without TGFβR1 knocked-down stimulated by bleomycin and quantification of grayscale value. h Q-PCR analysis of the relative mRNA expression levels of TGFβ, TGFβR1, Fn1, and Col1a1 in AF cells with bleomycin or/and LY364947, with or without TGFβR1 knocked-down stimulated by bleomycin. All qPCR data are calculated with Graphpad8.0 by ordinary one-way ANOVA test and presented as mean ± sd. from three experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001

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