Fig. 5
Histopathological examination of hDPSC-treated CXMDJ. a Hematoxylin and eosin (H&E) staining of the tibialis anterior (TA) and the gastrocnemius lateral (GL) muscle, and heart (left ventricular) from 1-year-old control DMD (12202MA, 13303MA, and 14102MA) and hDPSCs-DMD dogs (12205MA and 13304MA). Scale bar, 100 μm. b Muscle fiber areas (μm2) measured from the TA muscle of control and hDPSC-DMD dogs by H&E staining (n = 4). The average number of myofibers is described as the distribution. c Immunofluorescence analysis of the TA and gastrocnemius medialis (GM) muscles from the control DMD (12202MA, 14102MA, and 3903MA) and hDPSCs-DMD (12205MA and 13304MA) with anti-IgG antibodies (green) and anti-developmental myosin heavy chain antibodies (red) and DAPI (blue) staining. d Biodistribution of hDPSCs measured by Alu-PCR in the TA, extensor digitorum longus (EDL), extensor carpi ulnaris (ECU) muscle, diaphragm, heart (left ventricular, LV; right ventricular, RV), and lung tissue from repeated hDPSC-CXMDJ, 7 weeks after transplantation. N.D., not detected